The Establishment of a Tobramycin-Responsive Whole-Cell Micro-Biosensor Based on an Artificial Ribozyme Switch

In this study, a tobramycin concentration-dependent whole-cell micro-biosensor (tob-HHAz) was constructed by fusing aptamer with hammerhead ribozyme (HHR) from Schistosoma mansoni. The biosensor obtained integrating all the modules into one complete RNA sequence, which easily introduced E. coli without suffering harsh external environments. Three independent tobramycin-sensitive structures were identified via high-throughput screening in vivo and further verified vitro to undergo desired self-cleavage reaction. computation prediction of structure performed help analyze mechanisms various conformations performing qualitative rapid detection practical samples; two sensors exhibited high responsiveness spiked milk, limit around 40 nM, is below EU’s antibiotic maximum residual level. One provides linear range 30 650 nM minimum showed relatively good selectivity urine. This study first combined analysis optimize pivotal sensors. strategy enables researchers use artificial ribozyme-based biosensors not only for but also as generally applicable method substances living cells.